Gene Probes 1: A Practical Approach

The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals. Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes